BEER LAMBERT LAW

 The Beer-Lambert law is also referred to as Beer’s law.  It is a linear relationship that exists between the absorbance and the concentration of the absorbing species. Beer-Lambert law is mostly applied in chemical analysis and is used when one needs a proper understanding of the physical optics.  The Beer’s law is expressed in attenuation coefficient.  The beer’s law was discovered in 1729 by Pierre Bouguer.  The law is an attribute to Johann Heinrich Lambert.   The beer lambert law can be written as A=a (lambda) *b*c where A stands for measured absorbance, a (lambda) is wavelength, b is the path length and c is the analyte concentration.  When the wavelength passing through a spectrometer, the intensity of light is measured. The beer lambert law can be limited by nonlinearity like a scattered light as a result of particulate in a specified sample, changes in the refractive index, stray light and the shift of the chemical equilibria. In beer Lambert law, the experimental measurements are normally done in the terms of the transmittance.  Concentration and the solution length are both acceptable in beer lambert law.

Order Now